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Plant Tissue Culture Protocols
Orchid Culture Media
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K 4003
K 4128
P 0931
P 1056
P 6668
P 6793
M 5524 |
Knudson C modified orchid medium
Knudson C orchid medium, morel modification
Phytamax orchid maintenance medium without charcoal
Phytamax orchid medium with charcoal and banana powder
Phytamax orchid maintenance medium
Phytamax orchid multiplication medium
Murashige and Skoog basal salt mixture (MS)
(listed for purposes of comparison only)
|
| Component (mg/L) |
K 4003 |
K 4128 |
P 0931 |
P 1056 |
P 6668 |
P 6793 |
M 5524* |
| Aluminum chloride • 6H2O |
|
|
|
|
|
|
|
| Ammonium nitrate |
|
500.0 |
825.0 |
825.0 |
825.0 |
825.0 |
1650.0 |
| Ammonium phosphate monobasic |
|
|
|
|
|
|
|
| Ammonium sulfate |
500.0 |
500.0 |
|
|
|
|
|
| Boric acid |
0.056 |
|
3.1 |
3.1 |
3.1 |
3.1 |
6.2 |
| Calcium chloride anhydrous |
|
|
166.0 |
166.0 |
166.0 |
166.0 |
332.2 |
| Calcium nitrate |
694.4 |
347.2 |
|
|
|
|
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| Calcium phosphate tribasic |
|
|
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|
|
| Cobalt chloride • 6H2O |
|
|
0.0125 |
0.0125 |
0.0125 |
0.0125 |
0.025 |
| Cupric sulfate • 5H2O |
0.0624 |
|
0.0125 |
0.0125 |
0.0125 |
0.0125 |
0.025 |
| Na2-EDTA |
|
|
37.24 |
37.24 |
37.24 |
37.24 |
37.26 |
| ZnNa2-EDTA |
|
|
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| Ferric chloride • 6H2O |
|
|
|
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|
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| Ferric citrate |
|
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| Ferric sulfate |
|
|
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| Ferric tartrate • 2H2O |
|
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|
|
|
|
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| Ferrous sulfate • 7H2O |
25.0 |
25.0 |
27.85 |
27.85 |
27.85 |
27.85 |
27.8 |
| Magnesium sulfate |
122.125 |
122.125 |
90.35 |
90.35 |
90.35 |
90.35 |
180.7 |
| Manganese chloride • 4H2O |
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|
|
|
|
|
|
| Manganese sulfate • H2O |
5.682 |
5.682 |
8.45 |
8.45 |
8.45 |
8.45 |
16.9 |
| Molybdenum trioxide |
0.016 |
|
|
|
|
|
|
| Molybdic acid (sodium salt) • 2H2O |
|
|
0.125 |
0.125 |
0.125 |
0.125 |
0.25 |
| Nickel chloride • 6H2O |
|
|
|
|
|
|
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| Nickel sulfate • 6H2O |
|
|
|
|
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| Potassium chloride |
|
250.0 |
|
|
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| Potassium iodide |
|
|
0.415 |
0.415 |
0.415 |
0.415 |
0.83 |
| Potassium nitrate |
|
|
950.0 |
950.0 |
950.0 |
950.0 |
1900.0 |
| Potassium phosphate monobasic |
250.0 |
250.0 |
85.0 |
85.0 |
85.0 |
85.0 |
170.0 |
| Potassium sulfate |
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| Sodium nitrate |
|
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| Sodium phosphate monobasic |
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| Sodium sulfate |
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| Zinc nitrate • 6H2O |
|
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|
|
|
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| Zinc sulfate • 7H2O |
0.331 |
|
5.3 |
5.3 |
5.3 |
5.3 |
8.6 |
| Organics (mg/L) |
K 4003 |
K 4128 |
P 0931 |
P 1056 |
P 6668 |
P 6793 |
M 5524* |
| Activated charcoal |
|
|
|
2000.0 |
2000.0 |
|
|
| Adenine hemisulfate |
|
|
|
|
|
|
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| Agar |
|
|
|
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|
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| Banana powder (50% malto-dextrin) |
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|
30,000 |
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| 6-Benzylaminopurine (BA) |
|
|
|
|
|
2.0 |
|
| D-Biotin |
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6gamma-gamma-
Dimethylallylaminopurine |
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| Glycine (free base) |
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| Indole-3-acetic acid |
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| Indole-3-butyric acid |
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| Kinetin |
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| MES (free acid) |
|
|
1000.0 |
1000.0 |
1000.0 |
1000.0 |
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| myo-Inositol |
|
|
100.0 |
100.0 |
100.0 |
100.0 |
|
| alpha-Naphthaleneacetic acid |
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|
0.5 |
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| Nictotinic acid (free acid) |
|
|
1.0 |
1.0 |
1.0 |
0.5 |
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| Peptone |
|
|
2000.0 |
2000.0 |
2000.0 |
2000.0 |
|
| Pyridoxine • HCl |
|
|
1.0 |
1.0 |
1.0 |
0.5 |
|
| Sucrose |
20,000 |
20,000 |
20,000 |
20,000 |
20,000 |
20,000 |
|
| Thiamine • HCl |
|
|
10.0 |
10.0 |
10.0 |
1.0 |
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| |
K 4003 |
K 4128 |
P 0931 |
P 1056 |
P 6668 |
P 6793 |
M 5524* |
| Grams of powder to prepare 1 L |
21.6 |
22.0 |
25.3 |
57.3 |
27.3 |
25.3 |
4.3 |
| pH ± 0.5 at room temperature |
4.7 |
4.8 |
5.0 |
5.2 |
5.4 |
5.0 |
3.9 |
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*Composition for M 5524, Murashige and Skoog Basal Salt Mixture, listed for purposes of
comparison only.
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