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Plant Tissue Culture Protocols
Classic Plant Media
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| Product No. |
Description |
| C1416 |
Chu (N6) basal salt mixture |
| D6162 |
DKW/Juglans basal salt mixture |
| G5768 |
Gamborg's B-5 basal salt mixture |
| G5893 |
Gamborg's B-5 basal salt mixture with minimal organics |
| H2395 |
Hoagland's No. 2 basal salt mixture |
| M6774 |
McCown's woody plant basal salt mixture |
| M5524 |
Murashige and Skoog basal salt mixture (MS) |
| Q3376 |
Quoirin and Lepoivre basal salt mixture |
| S6765 |
Schenk and Hildebrandt basal salt mixture |
| W0876 |
White's basal salt mixture |
| Component (mg/L) |
C1416 |
D6162 |
G5768 |
G5893 |
H2395 |
M6774 |
M5524 |
Q3376 |
S6765 |
W0876 |
| Aluminum chloride • 6H2O |
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| Ammonium nitrate |
|
1416.0 |
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|
400.0 |
1650.0 |
400.0 |
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| Ammonium phosphate monobasic |
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|
115.03 |
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|
300.0 |
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| Ammonium sulfate |
463.0 |
|
134.0 |
134.0 |
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| Boric acid |
1.6 |
4.8 |
3.0 |
3.0 |
2.86 |
6.2 |
6.2 |
6.2 |
5.0 |
1.5 |
| Calcium chloride anhydrous |
125.33 |
112.5 |
113.24 |
113.24 |
|
72.5 |
332.2 |
|
151.0 |
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| Calcium nitrate |
|
1367.0 |
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|
656.4 |
386.0 |
|
833.77 |
|
200.0 |
| Calcium phosphate tribasic |
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| Cobalt chloride • 6H2O |
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|
0.025 |
0.025 |
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|
0.025 |
0.025 |
0.1 |
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| Cupric sulfate • 5H2O |
|
0.25 |
0.025 |
0.025 |
0.08 |
0.25 |
0.025 |
0.025 |
0.2 |
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| Na2-EDTA |
37.25 |
45.4 |
37.3 |
37.25 |
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37.3 |
37.26 |
37.3 |
20.0 |
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| ZnNa2-EDTA |
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| Ferric chloride • 6H2O |
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| Ferric citrate |
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| Ferric sulfate |
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|
2.5 |
| Ferric tartrate • 2H2O |
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|
5.32 |
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| Ferrous sulfate • 7H2O |
27.85 |
33.8 |
27.8 |
27.85 |
|
27.8 |
27.8 |
27.8 |
15.0 |
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| Magnesium sulfate |
90.37 |
361.49 |
122.09 |
122.09 |
240.76 |
180.7 |
180.7 |
175.79 |
195.4 |
360.0 |
| Manganese chloride • 4H2O |
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1.81 |
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| Manganese sulfate • H2O |
3.33 |
33.5 |
10.0 |
10.0 |
|
22.3 |
16.9 |
0.76 |
10.0 |
5.04 |
| Molybdenum trioxide |
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|
0.016 |
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| Molybdic acid (sodium salt) • 2H2O |
|
0.39 |
0.25 |
0.25 |
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0.25 |
0.25 |
0.25 |
0.1 |
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| Nickel chloride • 6H2O |
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| Nickel sulfate • 6H2O |
|
0.005 |
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| Potassium chloride |
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|
65.0 |
| Potassium iodide |
0.8 |
|
0.75 |
0.75 |
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|
0.83 |
0.08 |
1.0 |
0.75 |
| Potassium nitrate |
2830.0 |
|
2500.0 |
2500.0 |
606.6 |
|
1900.0 |
1800.0 |
2500.0 |
80.0 |
| Potassium phosphate monobasic |
400.0 |
265.0 |
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|
170.0 |
170.0 |
270.0 |
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| Potassium sulfate |
|
1559.0 |
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|
990.0 |
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| Sodium nitrate |
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| Sodium phosphate monobasic |
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|
130.5 |
130.5 |
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16.5 |
| Sodium sulfate |
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|
200.0 |
| Zinc nitrate • 6H2O |
|
17.0 |
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| Zinc sulfate • 7H2O |
1.5 |
|
2.0 |
2.0 |
0.22 |
8.6 |
8.6 |
8.6 |
1.0 |
2.67 |
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| Organics (mg/L) |
C1416 |
D6162 |
G5768 |
G5893 |
H2395 |
M6774 |
M5524 |
Q3376 |
S6765 |
W0876 |
| Activated charcoal |
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| Adenine hemisulfate |
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| Agar |
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| 6-Benzylaminopurine (BA) |
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| D-Biotin |
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6gamma-gamma-
Dimethylallylaminopurine |
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| Glycine (free base) |
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| Indole-3-acetic acid |
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| Indole-3-butyric acid |
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| Kinetin |
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| MES (free acid) |
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| myo-Inositol |
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100.0 |
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| alpha-Naphthaleneacetic acid |
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| Nictotinic acid (free acid) |
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1.0 |
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| Peptone |
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| Pyridoxine • HCl |
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1.0 |
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| Sucrose |
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| Thiamine • HCl |
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10.0 |
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| |
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C1416 |
D6162 |
G5768 |
G5893 |
H2395 |
M6774 |
M5524 |
Q3376 |
S6765 |
W0876 |
| Grams of powder to prepare 1 L |
4.0 |
5.2 |
3.1 |
3.2 |
1.6 |
2.3 |
4.3 |
3.6 |
3.2 |
0.934 |
| pH 1 0.5 at room temperature |
4.1 |
4.1 |
4.0 |
4.0 |
4.7 |
3.9 |
3.9 |
3.9 |
4.2 |
4.7 |
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