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Biological Buffers

MES

MES Specification Comparison PDF Printer friendly PDF version (100 Kb) FAQs and Live Chat Banner Image


Sigma-Aldrich® MES Physical Properties
Synonyms     2-(N-Morpholino)ethanesulfonic acid
    4-Morpholineethanesulfonic acid		 MES Image
Molecular Formula C6H13NO4S · xH2O CAS Number 4432-31-9 pKa (at 20) 6.16
Molecular Weight 195.24 (anhydrous basis) Useful pH range 5.5 - 6.7 pKa (at 25) 6.1
    pKa (at 37) 5.97
Sigma-Aldrich MES Specifications Comparison
  M3671 M8250 M5287 M2933 69890
Grade low moisture content, ≥99% (titration) ≥99.5% (titration) ≥99.5% (titration), pH 2.5 - 4.0
(20 °C, 0.5 M in H2O), SigmaUltra		 BPC, plant cell culture tested, ≥99.5% BioUltra,
≥99.5% (T)
Purity ≥99% ≥99.5% ≥99.5% ≥99.5% ≥99.5%
Reagent Testing
  M3671 M8250 M5287 M2933 69890
Enhanced Quality
Profile Buffers		 X X X X  
260NM     ≤0.025   ≤0.025
280NM     ≤0.020   ≤0.020
290NM ≤0.05 ≤0.05   ≤0.05  
IR X     X  
pH     2.5 - 4.0 (20 °C, 0.5 M in H2O)   2.5 - 4.0
(20 °C, 0.5 M in H2O)
Karl Fischer ≤1.0% ≤9% report result ≤9%  
Insoluble Matter     filter test   filter test
Residue on Ignition     ≤0.05%   ≤0.05%
Life Science Testing
  M3671 M8250 M5287 M2933 69890
Synthetic   X      
Endotoxin       ≤0.1 eu/mg  
SPC       ≤100 cfu/g  
Plant cell test       X  
RNAse       X  
DNAse       X  
Nickase       X  
Protease       X  
Trace Elemental Impurities
  M3671 M8250 M5287 M2933 69890
Sodium(Na)     ≤0.005%   ≤0.0050%
Chloride(Cl)     ≤0.005%   ≤0.0050%
Sulfate(SO4)         ≤0.0050%
Aluminum(Al)     ≤0.0005%   ≤0.0005%
Arsenic(As)     ≤0.0001%   ≤0.00001%
Barium(Ba)     ≤0.0005%   ≤0.0005%
Bismuth(Bi)     ≤0.0005%   ≤0.0005%
Calcium(Ca)     ≤0.002%   ≤0.0020%
Cadmium(Cd)     ≤0.0005%   ≤0.0005%
Cobalt(Co)     ≤0.0005%   ≤0.0005%
Chromium(Cr)     ≤0.0005%   ≤0.0005%
Copper(Cu)     ≤0.0005%   ≤0.0005%
Iron(Fe)     ≤0.0005% ≤0.0005% ≤0.0005%
Potassium(K)     ≤0.005%   ≤0.0050%
Lithium(Li)     ≤0.0005%   ≤0.0005%
Magnesium(Mg)     ≤0.0005%   ≤0.0005%
Manganese(Mn)     ≤0.0005%   ≤0.0005%
Molybdenum(Mo)     ≤0.0005%   ≤0.0005%
Nickel(Ni)     ≤0.0005%   ≤0.0005%
Lead(Pb) ≤0.0005% ≤0.0005% ≤0.0005% ≤0.0005% ≤0.0005%
Strontium(Sr)     ≤0.0005%   ≤0.0005%
Zinc(Zn)     ≤0.0005%   ≤0.0005%
 
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