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Biological Buffers
BIS-TRIS Propane
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| Sigma-Aldrich® BIS-TRIS Propane Physical Properties |
| Synonyms |
1,3-Bis[tris(hydroxymethyl)methylamino]propane |
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| Molecular Formula |
CH2[CH2NHC(CH2OH)3]2 |
Beilstein Registry |
1786109 |
Useful pH range |
6.3 - 9.5 |
| Molecular Weight |
282.33 |
MDL Number |
MFCD00004689 |
pKa (at 25) |
(1) 6.8, (2) 9.0 |
| CAS Number |
64431-96-5 |
EG/EC Number |
264-899-3 |
Melting Point |
164 - 165 °C(lit.) |
| Sigma-Aldrich BIS-TRIS Propane Specifications Comparison |
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B6755 |
B4679 |
B9410 |
| Grade |
≥99.0% (titration) |
BPC, cell culture tested, insect cell culture tested,
≥99.0% (titration) |
SigmaUltra, ≥99.0% (titration) |
| Purity |
≥99.0% |
≥99.0% |
>99.0% |
| Reagent Testing |
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B6755 |
B4679 |
B9410 |
Enhanced Quality
Profile Buffers |
X |
X |
X |
| 260NM |
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≤0.1 |
| 290NM |
≤0.05 |
≤0.05 |
≤0.05 |
| IR |
X |
X |
X |
| pH |
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10.0 - 12.0
(20 °C, 1 M in H2O) |
| Karl Fischer |
≤0.2% |
≤0.2% |
≤0.2% |
| Insoluble Matter |
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≤0.1% |
| Residue on Ignition |
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≤0.1% |
| Life Science Testing |
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B6755 |
B4679 |
B9410 |
| Endotoxin |
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≤1 eu/mg |
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| SPC |
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≤100 cfu/g |
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| Cell test |
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X |
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| RNAse |
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X |
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| DNAse |
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X |
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| Nickase |
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X |
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| Protease |
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X |
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| Proton NMR Spectrum |
≤0.5% Tris |
≤0.5% Tris |
≤0.5% Tris |
| Trace Elemental Impurities |
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B6755 |
B4679 |
B9410 |
| Ammonia(NH4) |
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≤0.05% |
| Sodium(Na) |
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≤0.005% |
| Phosphorus(P) |
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≤0.005% |
| Chloride(Cl) |
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≤0.05% |
| Sulfate(SO4) |
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≤0.05% |
| Aluminum(Al) |
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≤0.0005% |
| Calcium(Ca) |
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≤0.0005% |
| Copper(Cu) |
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≤0.0005% |
| Iron(Fe) |
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≤0.0005% |
| Potassium(K) |
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≤0.005% |
| Lead(Pb) |
≤0.0005% |
≤0.0005% |
≤0.001% |
| Magnesium(Mg) |
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≤0.0005% |
| Zinc(Zn) |
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≤0.0005% |
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